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Notice that the side chain has three methylene teams, so that though the terminal amino group shall be charged below physiological situations, the facet chain does have important hydrophobic character. Immunofluorescent staining of Human melanoma cells, utilizing Rabbit polyclonal to acetyl Lysine (ab21623) at 1:one hundred dilution. Incubate the membrane for 20 min and then wash with DMF to remove uncoupled amino acids.